Synchrotron SAXS
data from solutions of
Inhibitor of apoptosis-stimulating protein of p53 (iASPP(608-828)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, compact
in
25 mM Tris, 150 mM NaCl, 1 mM DTT, pH 8
were collected
on the
12.3.1 (SIBYLS) beam line
at the Advanced Light Source (ALS) storage ring
(Berkeley, CA, USA)
using a Pilatus3 X 2M detector
at a sample-detector distance of 2.1 m and
at a wavelength of λ = 0.103 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample
was injected at a 0.50 ml/min flow rate
onto a Shodex KW-800 series column
at 20°C.
800 successive
3 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
|
Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor)
(iASPP)
|
Mol. type |
|
Protein |
Organism |
|
Homo sapiens |
Olig. state |
|
Monomer |
Mon. MW |
|
24.9 kDa |
|
UniProt |
|
Q8WUF5 (608-828) |
Sequence |
|
FASTA |
|
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
(PP-1cα)
|
Mol. type |
|
Protein |
Organism |
|
Homo sapiens |
Olig. state |
|
Monomer |
Mon. MW |
|
37.5 kDa |
|
UniProt |
|
P62136 (1-330) |
Sequence |
|
FASTA |
|
PDB code |
|
6DCX |
|
PDB code |
|
6DCX |
|
PDB code |
|
6DCX |
|
|