Flexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis.

Zhou Y, Millott R, Kim HJ, Peng S, Edwards RA, Skene-Arnold T, Hammel M, Lees-Miller SP, Tainer JA, Holmes CFB, Glover JNM, Structure 27(10):1485-1496.e4 (2019) Europe PMC

SASDG76 – Inhibitor of apoptosis-stimulating protein of p53 (iASPP(608-828)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, compact

Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor)
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
MWexperimental 58 kDa
MWexpected 62 kDa
VPorod 157 nm3
log I(s) 1.78×103 1.78×102 1.78×101 1.78×100
Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) Serine/threonine-protein phosphatase PP1-alpha catalytic subunit small angle scattering data  s, nm-1
ln I(s)
Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) Serine/threonine-protein phosphatase PP1-alpha catalytic subunit Guinier plot ln 1.78×103 Rg: 3.1 nm 0 (3.1 nm)-2 s2
(sRg)2I(s)/I(0)
Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) Serine/threonine-protein phosphatase PP1-alpha catalytic subunit Kratky plot 1.104 0 √3 sRg
p(r)
Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) Serine/threonine-protein phosphatase PP1-alpha catalytic subunit pair distance distribution function Rg: 3.2 nm 0 Dmax: 11.3 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) Serine/threonine-protein phosphatase PP1-alpha catalytic subunit BILBOMD model
Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) Serine/threonine-protein phosphatase PP1-alpha catalytic subunit BILBOMD model
Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) Serine/threonine-protein phosphatase PP1-alpha catalytic subunit BILBOMD model
Synchrotron SAXS data from solutions of Inhibitor of apoptosis-stimulating protein of p53 (iASPP(608-828)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, compact in 25 mM Tris, 150 mM NaCl, 1 mM DTT, pH 8 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS) storage ring (Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample was injected at a 0.50 ml/min flow rate onto a Shodex KW-800 series column at 20°C. 800 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) (iASPP)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   24.9 kDa
 
UniProt   Q8WUF5 (608-828)
Sequence   FASTA
 
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (PP-1cα)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   37.5 kDa
 
UniProt   P62136 (1-330)
Sequence   FASTA
 
PDB code   6DCX
 
PDB code   6DCX
 
PDB code   6DCX