| MWexperimental | 65 | kDa |
| MWexpected | 62 | kDa |
| VPorod | 180 | nm3 |
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log I(s)
2.23×103
2.23×102
2.23×101
2.23×100
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s, nm-1
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Flexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis.
Zhou Y, Millott R, Kim HJ, Peng S, Edwards RA, Skene-Arnold T, Hammel M, Lees-Miller SP, Tainer JA, Holmes CFB, Glover JNM, Structure 27(10):1485-1496.e4 (2019) Europe PMCSASDG86 – Inhibitor of apoptosis-stimulating protein of p53 (iASPP(608-828)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, extended
Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor)Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
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Fits and models
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Synchrotron SAXS
data from solutions of
Inhibitor of apoptosis-stimulating protein of p53 (iASPP(608-828)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, extended
in
25 mM Tris, 150 mM NaCl, 1 mM DTT, pH 8
were collected
on the
12.3.1 (SIBYLS) beam line
at the Advanced Light Source (ALS) storage ring
(Berkeley, CA, USA)
using a Pilatus3 X 2M detector
at a sample-detector distance of 2.1 m and
at a wavelength of λ = 0.103 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample
was injected at a 0.50 ml/min flow rate
onto a Shodex KW-800 series column
at 20°C.
800 successive
3 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Sample injection volume = UNKNOWN |
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