| MWexperimental | 57 | kDa |
| MWexpected | 64 | kDa |
| VPorod | 144 | nm3 |
|
log I(s)
1.77×103
1.77×102
1.77×101
1.77×100
|
s, nm-1
|
Flexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis.
Zhou Y, Millott R, Kim HJ, Peng S, Edwards RA, Skene-Arnold T, Hammel M, Lees-Miller SP, Tainer JA, Holmes CFB, Glover JNM, Structure 27(10):1485-1496.e4 (2019) Europe PMCSASDG96 – Apoptosis-stimulating protein 2 of p53 (ASPP2(905-1128)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, compact
Serine/threonine-protein phosphatase PP1-alpha catalytic subunitApoptosis-stimulating of p53 protein 2
|
|
|
Fits and models
|
|
|
Synchrotron SAXS
data from solutions of
Apoptosis-stimulating protein 2 of p53 (ASPP2(905-1128)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, compact
in
25 mM Tris, 150 mM NaCl, 1 mM DTT, pH 8
were collected
on the
12.3.1 (SIBYLS) beam line
at the Advanced Light Source (ALS) storage ring
(Berkeley, CA, USA)
using a Pilatus3 X 2M detector
at a sample-detector distance of 2.1 m and
at a wavelength of λ = 0.103 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample
was injected at a 0.50 ml/min flow rate
onto a Shodex KW-800 series column
at 20°C.
800 successive
3 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Sample injection volume = UNKNOWN |
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||