Cooperativity of UBA5-UFM1 complex formation

Sebastian Fuchs.

SASDGH6 – Ubiquitin activating enzyme 5 (1.0 mg/ml)

Ubiquitin-like modifier-activating enzyme 5
MWI(0) 58 kDa
MWexpected 68 kDa
VPorod 82 nm3
log I(s) 4.90×10-3 4.90×10-4 4.90×10-5 4.90×10-6
Ubiquitin-like modifier-activating enzyme 5 small angle scattering data  s, nm-1
ln I(s)
Ubiquitin-like modifier-activating enzyme 5 Guinier plot ln 4.90×10-3 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
Ubiquitin-like modifier-activating enzyme 5 Kratky plot 1.104 0 3 sRg
p(r)
Ubiquitin-like modifier-activating enzyme 5 pair distance distribution function Rg: 2.8 nm 0 Dmax: 10 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Ubiquitin-like modifier-activating enzyme 5 SASREF model
Ubiquitin-like modifier-activating enzyme 5 SASREF model
Ubiquitin-like modifier-activating enzyme 5 SASREF model

Synchrotron SAXS data from solutions of Ubiquitin activating enzyme 5 (1.0 mg/ml) in 20 mM Tris, 150 mM NaCl, 2 mM DTT, pH 7.5 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123982 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.04 mg/ml was measured at 15°C. 50 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Ubiquitin-like modifier-activating enzyme 5 (UBA5)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   34.2 kDa
 
UniProt   Q9GZZ9 (57-346)
Sequence   FASTA
 
PDB code   5IAA