Apolipoprotein E project

Lucas Kraft.

SASDGU7 – Truncated Apolipoprotein E4 (1-191) bound to 330 µg/mL heparin

Apolipoprotein E4 (1-191)
MWexperimental 33 kDa
MWexpected 38 kDa
log I(s) 1.10×10-1 1.10×10-2 1.10×10-3 1.10×10-4
Heparin Apolipoprotein E4 (1-191) small angle scattering data  s, nm-1
ln I(s)
Heparin Apolipoprotein E4 (1-191) Guinier plot ln 1.10×10-1 Rg: 3.1 nm 0 (3.1 nm)-2 s2
Heparin Apolipoprotein E4 (1-191) Kratky plot 1.104 0 3 sRg
Heparin Apolipoprotein E4 (1-191) pair distance distribution function Rg: 3.1 nm 0 Dmax: 10.5 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Truncated Apolipoprotein E4 (1-191) bound to 330 µg/mL heparin in 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH 8 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 20°C. 28 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

ApoE4 (1-191) + 330 µg/mL heparin. Molecular weight estimates are inaccurate due to heparin heterogeneity.

Mol. type   Other
Olig. state   Monomer
Mon. MW   15 kDa
Chemical formula
Apolipoprotein E4 (1-191) (ApoE4)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   22.6 kDa
UniProt   P02649 (19-209)
Sequence   FASTA