The guide sRNA sequence determines the activity level of box C/D RNPs.

Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T, Elife 9 (2020) Europe PMC

SASDGV2 – The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Nop5, 1H L7Ae, 1H st-sR26 RNA, 1H substrate D' and 2H Fibrillarin (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase
50S ribosomal protein L7Ae
NOP5/NOP56 related protein
pyrococcus furiosus sR26 stabilized construct
Pyrococcus furiosus sR26 substrate D'
MWexperimental 200 kDa
MWexpected 200 kDa
VPorod 87 nm3
log I(s) 6.43×10-1 6.43×10-2 6.43×10-3 6.43×10-4
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' small angle scattering data  s, nm-1
ln I(s)
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Guinier plot ln 6.43×10-1 Rg: 4.9 nm 0 (4.9 nm)-2 s2
(sRg)2I(s)/I(0)
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot 1.104 0 3 sRg
p(r)
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' pair distance distribution function Rg: 5.3 nm 0 Dmax: 16 nm

Data validation


There are no models related to this curve.

SANS data from solutions of the Pyrococcus furiosus Box C/D sRNP complex reconsituted with 1H Nop5, 1H L7Ae, 1H st-sR26 RNA, 1H substrate D' and 2H Fibrillarin in 50 mM phosphate 500 mM NaCl 42%D2O, pH 6.6 were collected on KWS1 (FRM2; Munich, Germany) using a 6Li-Scintillator 1 mm thickness + photomultiplier detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.499 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 8.90 mg/ml was measured at 55°C. One 7200.001 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase (Fibrillarin)
Mol. type   Protein
Organism   Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Olig. state   Dimer
Mon. MW   25.8 kDa
 
UniProt   Q8U4M2 (1-227)
Sequence   FASTA
 
50S ribosomal protein L7Ae (L7Ae)
Mol. type   Protein
Organism   Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Olig. state   Dimer
Mon. MW   13.4 kDa
 
UniProt   Q8U160 (1-123)
Sequence   FASTA
 
NOP5/NOP56 related protein (Nop5)
Mol. type   Protein
Organism   Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Olig. state   Dimer
Mon. MW   46.8 kDa
 
UniProt   Q8U4M1 (1-402)
Sequence   FASTA
 
pyrococcus furiosus sR26 stabilized construct (st-sR26)
Mol. type   RNA
Organism   Pyrococcus furiosus
Olig. state   Monomer
Mon. MW   23.5 kDa
Sequence   FASTA
 
Pyrococcus furiosus sR26 substrate D' (Substrate D')
Mol. type   RNA
Organism   Pyrococcus furiosus
Olig. state   Monomer
Mon. MW   4.4 kDa
Sequence   FASTA