Interaction of the tetratricopeptide repeat domain of aryl hydrocarbon receptor-interacting protein-like 1 with the regulatory Pγ subunit of phosphodiesterase 6.

Yadav RP, Boyd K, Yu L, Artemyev NO, J Biol Chem 294(43):15795-15807 (2019) Europe PMC

SASDGX4 – Aryl-hydrocarbon-interacting protein-like 1

Aryl-hydrocarbon-interacting protein-like 1(1-316)
MWexperimental 37 kDa
MWexpected 37 kDa
VPorod 60 nm3
log I(s) 5.68×101 5.68×100 5.68×10-1 5.68×10-2
Aryl-hydrocarbon-interacting protein-like 1(1-316) small angle scattering data  s, nm-1
ln I(s)
Aryl-hydrocarbon-interacting protein-like 1(1-316) Guinier plot ln 5.69×101 Rg: 2.6 nm 0 (2.6 nm)-2 s2
(sRg)2I(s)/I(0)
Aryl-hydrocarbon-interacting protein-like 1(1-316) Kratky plot 1.104 0 3 sRg
p(r)
Aryl-hydrocarbon-interacting protein-like 1(1-316) pair distance distribution function Rg: 2.7 nm 0 Dmax: 9.1 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of the aryl-hydrocarbon-interacting protein-like 1 in 50 mM Tris, 100 mM NaCl, 2.5 % glycerol and 6 mM DTT, pH 7.5 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.5 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 250.00 μl sample at 10 mg/ml was injected onto a GE Superdex 75 Increase 10/300 column . The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. Number of frames = UNKNOWN. Flow rate = UNKNOWN

Aryl-hydrocarbon-interacting protein-like 1(1-316) (AIPL1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   37.1 kDa
 
UniProt   Q9NZN9
Sequence   FASTA