Apolipoprotein E project

Lucas Kraft.

SASDGX7 – Truncated Apolipoprotein E4 (1-191) bound to 4 µg/mL heparin

Heparin
Apolipoprotein E4 (1-191)
MWexperimental 24 kDa
MWexpected 38 kDa
log I(s) 7.60×10-2 7.60×10-3 7.60×10-4 7.60×10-5
Heparin Apolipoprotein E4 (1-191) small angle scattering data  s, nm-1
ln I(s)
Heparin Apolipoprotein E4 (1-191) Guinier plot ln 7.60×10-2 Rg: 2.4 nm 0 (2.4 nm)-2 s2
(sRg)2I(s)/I(0)
Heparin Apolipoprotein E4 (1-191) Kratky plot 1.104 0 3 sRg
p(r)
Heparin Apolipoprotein E4 (1-191) pair distance distribution function Rg: 2.5 nm 0 Dmax: 8 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of Truncated Apolipoprotein E4 (1-191) bound to 4 µg/mL heparin in 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH 8 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 20°C. 28 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

ApoE4 (1-191) + 4 µg/mL heparin. Molecular weight estimates are inaccurate due to heparin heterogeneity.

Heparin
Mol. type   Other
Olig. state   Monomer
Mon. MW   15 kDa
Chemical formula
 
Apolipoprotein E4 (1-191) (ApoE4)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   22.6 kDa
 
UniProt   P02649 (19-209)
Sequence   FASTA