| MWexperimental | 265 | kDa |
| MWexpected | 194 | kDa |
| VPorod | 437 | nm3 |
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log I(s)
8.00×10-3
8.00×10-4
8.00×10-5
8.00×10-6
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s, nm-1
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Mechanism of Crosstalk between the LSD1 Demethylase and HDAC1 Deacetylase in the CoREST Complex.
Song Y Dagil L, Fairall L, Robertson N, Wu M, Ragan TJ, Savva CG, Saleh A, Morone N, Kunze MBA, Jamieson AG, Cole PA, Hansen DF, Schwabe JWR, Cell Rep 30(8):2699-2711.e8 (2020) Europe PMCSASDH45 – Histone Deacetylase 1 (HDAC1) and Lysine-specific Demethylase 1 (LSD1) in the complex with CoREST
Histone deacetylase 1Lysine-specific histone demethylase 1A
REST corepressor 1
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Fits and models
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Synchrotron SAXS data from solutions of histone deacetylase 1 (HDAC1) and lysine-specific demethylase 1 (LSD1) in the complex with CoREST in 25 mM Tris/Cl, 50 mM potassium acetate and 0.5 mM TCEP, pH 7.5 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.13 mg/ml was measured at 25°C. 30 successive 0.160 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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