| MWexperimental | 265 | kDa |
| MWexpected | 194 | kDa |
| VPorod | 437 | nm3 |
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log I(s)
8.00×10-3
8.00×10-4
8.00×10-5
8.00×10-6
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s, nm-1
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Mechanism of Crosstalk between the LSD1 Demethylase and HDAC1 Deacetylase in the CoREST Complex.
Song Y, Dagil L, Fairall L, Robertson N, Wu M, Ragan TJ, Savva CG, Saleh A, Morone N, Kunze MBA, Jamieson AG, Cole PA, Hansen DF, Schwabe JWR, Cell Rep 30(8):2699-2711.e8 (2020) Europe PMCSASDH45 – Histone Deacetylase 1 (HDAC1) and Lysine-specific Demethylase 1 (LSD1) in the complex with CoREST
Histone deacetylase 1Lysine-specific histone demethylase 1A
REST corepressor 1
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Fits and models
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Synchrotron SAXS data from solutions of histone deacetylase 1 (HDAC1) and lysine-specific demethylase 1 (LSD1) in the complex with CoREST in 25 mM Tris/Cl, 50 mM potassium acetate and 0.5 mM TCEP, pH 7.5 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.13 mg/ml was measured at 25°C. 30 successive 0.160 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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