Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ1 RNA Riboswitches.

Gong Z, Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C, J Mol Biol 432(16):4523-4543 (2020) Europe PMC

SASDHA8 – Wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis, holo-state high-temperature sampling - Tte-WT-holo-HT

wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis
MWexperimental 11 kDa
MWexpected 11 kDa
VPorod 10 nm3
log I(s) 1.99×101 1.99×100 1.99×10-1 1.99×10-2
wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis small angle scattering data  s, nm-1
ln I(s)
wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis Guinier plot ln 1.99×101 Rg: 1.5 nm 0 (1.5 nm)-2 s2
(sRg)2I(s)/I(0)
wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis Kratky plot 1.104 0 3 sRg
Dmax: 6.5 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of Tte-WT-holo-HT in 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH 6.5 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Pilatus 1M detector at a wavelength of λ = 0.1037 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). 20 successive 1 second frames were collected at 60°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Holo state sampling at 333K

wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis (Tte-WT)
Mol. type   RNA
Organism   Caldanaerobacter subterraneus subsp. tengcongensis
Olig. state   Monomer
Mon. MW   10.7 kDa
Sequence   FASTA