The BRCA2-MEILB2-BRME1 complex governs meiotic recombination and impairs the mitotic BRCA2-RAD51 function in cancer cells

Zhang J, Gurusaran M, Fujiwara Y, Zhang K, Echbarthi M, Vorontsov E, Guo R, Pendlebury D, Alam I, Livera G, Emmanuelle M, Wang P, Nandakumar J, Davies O, Shibuya H, Nature Communications 11(1) (2020) DOI

SASDHA9 – Meiotic localizer of BRCA2 - MEILB2a1+2 dimer

Meiotic localizer of BRCA2
MWexperimental 48 kDa
MWexpected 26 kDa
VPorod 88 nm3
log I(s) 4.09×10-2 4.09×10-3 4.09×10-4 4.09×10-5
Meiotic localizer of BRCA2 small angle scattering data  s, nm-1
ln I(s)
Meiotic localizer of BRCA2 Guinier plot ln 4.10×10-2 Rg: 4.6 nm 0 (4.6 nm)-2 s2
(sRg)2I(s)/I(0)
Meiotic localizer of BRCA2 Kratky plot 1.104 0 3 sRg
p(r)
Meiotic localizer of BRCA2 pair distance distribution function Rg: 4.8 nm 0 Dmax: 16 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Meiotic localizer of BRCA2 DAMMIF model
Meiotic localizer of BRCA2 DAMMIF model

Synchrotron SAXS data from solutions of the meiotic localizer of BRCA2 (MEILB2a1+2 dimer) in 20 mM Tris pH 8.0, 150 mM KCl were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a wavelength of λ = 0.095 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: An 80 μl sample at 15 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 25 successive 3 second frames were collected through the sample elution peak. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of an appropriate solvent-blank from the SEC-elution was subtracted.

Meiotic localizer of BRCA2 (MEILB2)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Dimer
Mon. MW   12.8 kDa
 
UniProt   A0A3T0ZJB3 (18-122)
Sequence   FASTA