Synchrotron SAXS data from solutions of the N-terminal domain of Wag3 in 20 mM Tris pH 7.5, 150 mM NaCl, 10% glycerol, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 30.00 μl sample at 4.4 mg/ml was injected at a 1.00 ml/min flow rate onto a Agilent Bio SEC-3, 300 Å column at 20°C. 1500 successive 1 second frames were collected through the elution profile. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of an appropriate solvent blank solvent-blank was subtracted from the corresponding sample scattering measured through the SEC elution peak.
s, nm-1
