Synchrotron SAXS data from solutions of Histatin 5 in 20 mM Tris, 150 mM NaCl, pH 7 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.26 mg/ml was measured at 25°C. 30 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The data are the combined results from nine replicates and are normalised to protein concentration. The concentration independent MW estimate is 3.8 kDa in the credibility interval 3.3-4.1 kDa.