Pseudo-RNA-Binding Domains Mediate RNA Structure Specificity in Upstream of N-Ras.

Hollmann NM, Jagtap PKA, Masiewicz P, Guitart T, Simon B, Provaznik J, Stein F, Haberkant P, Sweetapple LJ, Villacorta L, Mooijman D, Benes V, Savitski MM, Gebauer F, Hennig J, Cell Rep 32(3):107930 (2020) Europe PMC

SASDHK7 – Upstream of N-ras, isoform A, CSD 7 and 8 from Drosophila melanogaster

Upstream of N-ras, isoform A
MWexperimental 19 kDa
MWexpected 19 kDa
VPorod 28 nm3
log I(s) 2.54×103 2.54×102 2.54×101 2.54×100
Upstream of N-ras, isoform A small angle scattering data  s, nm-1
ln I(s)
Upstream of N-ras, isoform A Guinier plot ln 2.54×103 Rg: 1.8 nm 0 (1.8 nm)-2 s2
(sRg)2I(s)/I(0)
Upstream of N-ras, isoform A Kratky plot 1.104 0 √3 sRg
Dmax: 6 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Unr CSD 7 and 8 in 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.80 mg/ml was measured at 25°C. 20 successive 0.195 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Upstream of N-ras, isoform A (Unr)
Mol. type   Protein
Organism   Drosophila melanogaster
Olig. state   Monomer
Mon. MW   18.7 kDa
 
UniProt   Q9VSK3 (756-922)
Sequence   FASTA