Amyloid Evolution: Antiparallel Replaced by Parallel.

Zanjani AAH, Reynolds NP, Zhang A, Schilling T, Mezzenga R, Berryman JT, Biophys J (2020) Europe PMC

SASDHK8 – Ile-Leu-Gln-Ile-Asn-Ser (ILQINS) hexapeptide self-assembly (2019-dataset)

Ile-Leu-Gln-Ile-Asn-Ser peptide
MWexperimental 600 kDa
MWexpected 1 kDa
log I(s)
Ile-Leu-Gln-Ile-Asn-Ser peptide small angle scattering data  s, nm-1
Ile-Leu-Gln-Ile-Asn-Ser peptide Kratky plot 0 3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Ile-Leu-Glu-Ile-Asn-Ser (ILQINS) hexapeptide self-assemblies suspended in pure (MQ, 18 MΩ) water, pH 7, were collected on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 1.6 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 25°C. 15 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The SAXS/WAXS profile shows a solution of ILQINS peptide measured during the process of aggregation. The beam width is smaller than estimated size of (some) aggregates, so the calculation of Rg or of the self-assembled polymer molecular weight is not valid; the assemblies of the 0.7 kDa ILQINS peptide units are in the order of several hundred(s)/thousands of kDa. Related SASBDB entry: SASDHQ5.

Ile-Leu-Gln-Ile-Asn-Ser peptide (ILQINS)
Mol. type   Protein
Organism   synthetic construct
Olig. state   Unknown
Mon. MW   0.7 kDa
Sequence   FASTA