Structural Mimicry Drives HIV-1 Rev-Mediated HERV-K Expression

O'Carroll I, Fan L, Kroupa T, McShane E, Theodore C, Yates E, Kondrup B, Ding J, Martin T, Rein A, Wang Y, Journal of Molecular Biology 432(24):166711 (2020) DOI

SASDHL9 – Full-length HERV-K Rec response element

Full-length HERV-K Rec response element
MWexperimental 132 kDa
MWexpected 141 kDa
VPorod 454 nm3
log I(s) 4.00×10-1 4.00×10-2 4.00×10-3 4.00×10-4
Full-length HERV-K Rec response element small angle scattering data  s, nm-1
ln I(s)
Full-length HERV-K Rec response element Guinier plot ln 4×10-1 Rg: 7.5 nm 0 (7.5 nm)-2 s2
(sRg)2I(s)/I(0)
Full-length HERV-K Rec response element Kratky plot 1.104 0 √3 sRg
p(r)
Full-length HERV-K Rec response element pair distance distribution function Rg: 8 nm 0 Dmax: 28.2 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of full-length HERV-K Rec response element in 10mM Tris-HCl, 100mM KCl, and 0.5mM MgCl₂, pH 8 were collected on the 12ID-B SAXS/WAXS beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus 2M detector at a sample-detector distance of 1.9 m and at a wavelength of λ = 0.0932 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.8 and 1.3 mg/ml were measured at 20°C. 30 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.

Volume of correlation, Vc, was used to estimate the molecular weight.

Full-length HERV-K Rec response element (FL RcRE)
Mol. type   RNA
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   140.5 kDa
Sequence   FASTA