Architecture and functional dynamics of the pentafunctional AROM complex

Arora Verasztó H, Logotheti M, Albrecht R, Leitner A, Zhu H, Hartmann M, Nature Chemical Biology (2020) DOI

SASDHP8 – Pentafunctional AROM polypeptide from Chaetomium thermophilum

Pentafunctional AROM polypeptide
MWexperimental 340 kDa
MWexpected 345 kDa
VPorod 623 nm3
log I(s) 6.68×10-2 6.68×10-3 6.68×10-4 6.68×10-5
Pentafunctional AROM polypeptide small angle scattering data  s, nm-1
ln I(s)
Pentafunctional AROM polypeptide Guinier plot ln 6.68×10-2 Rg: 5.4 nm 0 (5.4 nm)-2 s2
(sRg)2I(s)/I(0)
Pentafunctional AROM polypeptide Kratky plot 1.104 0 3 sRg
Dmax: 17.5 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of the pentafunctional AROM polypeptide from Chaetomium thermophilum in 20 mM HEPES, 150 mM NaCl. 2 mM TCEP, 1% sucrose, pH 8 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 3.9 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample at 15 mg/ml was injected onto a Shodex KW403 column at 20°C (sample injection concentration, unknown; column flow rate, unknown; X-ray exposure time unknown). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Pentafunctional AROM polypeptide (AROM complex)
Mol. type   Protein
Organism   Chaetomium thermophilum
Olig. state   Dimer
Mon. MW   172.6 kDa
 
UniProt   G0S061 (1-1581)
Sequence   FASTA