Assembly of the mitochondrial Complex I assembly complex suggests a regulatory role for deflavination.

Giachin G, Jessop M, Bouverot R, Acajjaoui S, Saidi M, Chretien A, Bacia-Verloop M, Signor L, Mas PJ, Favier A, Borel Meneroud E, Hons M, Hart DJ, Kandiah E, Boeri Erba E, Buisson A, Leonard G, Gutsche I, Soler-Lopez M, Angew Chem Int Ed Engl (2020) Europe PMC

SASDHV4 – Mitochondrial complex I assembly factor, NDUFAF1

Complex I intermediate-associated protein 30, mitochondrial
MWI(0) 32 kDa
MWexpected 35 kDa
VPorod 82 nm3
log I(s) 2.97×101 2.97×100 2.97×10-1 2.97×10-2
Complex I intermediate-associated protein 30, mitochondrial small angle scattering data  s, nm-1
ln I(s)
Complex I intermediate-associated protein 30, mitochondrial Guinier plot ln 2.98×101 Rg: 3.3 nm 0 (3.3 nm)-2 s2
(sRg)2I(s)/I(0)
Complex I intermediate-associated protein 30, mitochondrial Kratky plot 1.104 0 √3 sRg
p(r)
Complex I intermediate-associated protein 30, mitochondrial pair distance distribution function Rg: 3.1 nm 0 Dmax: 9.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Complex I intermediate-associated protein 30, mitochondrial DAMMIN model
Synchrotron SAXS data from solutions of NDUFAF1 in 25 mM HEPES, 250 mM NaCl, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.6 and 3.5 mg/ml were measured at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

NDUFAF1 is a mitochondrial protein involved in the correct assembly of Complex I.

Complex I intermediate-associated protein 30, mitochondrial (NDUFAF1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   35.1 kDa
 
UniProt   Q9Y375 (25-327)
Sequence   FASTA