data from solutions of
Monomeric Jagged1 high-affinity full ectodomain
20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH 7.4
B21 beam line
at the Diamond Light Source storage ring
using a Eiger 4M detector
at a sample-detector distance of 4.0 m and
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample
at 5.9 mg/ml was injected
onto a column
3 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The expected molecular weight of the protein quoted above excludes the contribution of N- and O-linked glycans. The total expected molecular weight of the protein in a monomeric state is 130 kDa (protein, 115 kDa plus glycans, 15 kDa).
X-ray wavelength: UNKNOWN. Temperature: UNKNOWN. SEC column: UNKNOWN. SEC flow-rate: UNKNOWN.