data from solutions of
Jagged1 high-affinity full ectodomain 21 µM
20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH 7.4
B21 beam line
at the Diamond Light Source storage ring
using a Eiger 4M detector
at a sample-detector distance of 4.0 m and
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 2.94 mg/ml was measured
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The expected molecular weight of the protein quoted above excludes the contribution of N- and O-linked glycans. The total expected molecular weight of the protein in a monomeric state is 130 kDa (protein, 115 kDa plus glycans, 15 kDa).
X-ray wavelength: UNKNOWN. Exposure time = UNKNOWN.