Notch-Jagged signaling complex defined by an interaction mosaic

Matthieu Zeronian.

SASDJ78 – Jagged1 high-affinity full ectodomain 42 µM

Protein jagged-1
MWexperimental 303 kDa
MWexpected 115 kDa
VPorod 900 nm3
log I(s) 1.55×100 1.55×10-1 1.55×10-2 1.55×10-3
Protein jagged-1 small angle scattering data  s, nm-1
ln I(s)
Protein jagged-1 Guinier plot ln 1.55×100 Rg: 10.2 nm 0 (10.2 nm)-2 s2
(sRg)2I(s)/I(0)
Protein jagged-1 Kratky plot 1.104 0 3 sRg
p(r)
Protein jagged-1 pair distance distribution function Rg: 11.0 nm 0 Dmax: 43 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of Jagged1 high-affinity full ectodomain 42 µM in 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH 7.4 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 4.0 m and (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.88 mg/ml was measured at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The expected molecular weight of the protein quoted above excludes the contribution of N- and O-linked glycans. The total expected molecular weight of the protein in a monomeric state is 130 kDa (protein, 115 kDa plus glycans, 15 kDa). X-ray wavelength: UNKNOWN. Exposure time = UNKNOWN. Temperature: UNKNOWN.

Protein jagged-1
Mol. type   Protein
Organism   Mus musculus
Olig. state   Unknown
Mon. MW   114.6 kDa
 
UniProt   Q9QXX0 (31-1067)
Sequence   FASTA