Synchrotron SAXS data from solutions of the DNA repair protein XRCC1 - DNA Ligase IIIα complex in 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 5 mM MgCl₂, 0.2 mM PMSF, 1 mM benzamidine, were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 4 mg/ml was injected at a 0.50 ml/min flow rate onto a Shodex LW-803 column at 20°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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DNA repair protein XRCC1
(XRCC1)
|
Mol. type |
|
Protein |
Organism |
|
Homo sapiens |
Olig. state |
|
Unknown |
Mon. MW |
|
69.5 kDa |
|
UniProt |
|
P18887
(1-633)
|
Sequence |
|
FASTA |
|
DNA ligase 3 (DNA ligase III alpha)
(LigIIIα)
|
Mol. type |
|
Protein |
Organism |
|
Homo sapiens |
Olig. state |
|
Other |
Mon. MW |
|
102.7 kDa |
|
UniProt |
|
P49916
(88-1009)
|
Sequence |
|
FASTA |
|
|