Notch-Jagged signaling complex defined by an interaction mosaic

Matthieu Zeronian.

SASDJA8 – Jagged1 EGF8-11 115 µM

Protein jagged-1 EGF8-11
MWexperimental 30 kDa
MWexpected 21 kDa
VPorod 34 nm3
log I(s) 1.13×10-1 1.13×10-2 1.13×10-3 1.13×10-4
Protein jagged-1 EGF8-11 small angle scattering data  s, nm-1
ln I(s)
Protein jagged-1 EGF8-11 Guinier plot ln 1.13×10-1 Rg: 3.1 nm 0 (3.1 nm)-2 s2
Protein jagged-1 EGF8-11 Kratky plot 1.104 0 3 sRg
Protein jagged-1 EGF8-11 pair distance distribution function Rg: 3.3 nm 0 Dmax: 11.5 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Jagged1 EGF8-11 115 µM in 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH 7.4 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 4.0 m and (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.50 mg/ml was measured at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The expected molecular weight of the protein quoted above excludes the contribution of O-linked glycans. The total expected molecular weight of the protein in a monomeric state is 22 kDa (protein, 21 kDa plus glycans, 1 kDa). X-ray wavelength: UNKNOWN. Exposure time = UNKNOWN.

Protein jagged-1 EGF8-11
Mol. type   Protein
Organism   Mus musculus
Olig. state   Unknown
Mon. MW   20.6 kDa
UniProt   Q9QXX0 (487-665)
Sequence   FASTA