Notch-Jagged signaling complex defined by an interaction mosaic

Matthieu Zeronian.

SASDJE8 – Jagged1 cysteine-rich domain (CRD) 83 µM

Protein jagged-1 cysteine-rich domain
MWexperimental 30 kDa
MWexpected 24 kDa
VPorod 59 nm3
log I(s) 8.88×10-2 8.88×10-3 8.88×10-4 8.88×10-5
Protein jagged-1 cysteine-rich domain small angle scattering data  s, nm-1
ln I(s)
Protein jagged-1 cysteine-rich domain Guinier plot ln 8.88×10-2 Rg: 2.3 nm 0 (2.3 nm)-2 s2
(sRg)2I(s)/I(0)
Protein jagged-1 cysteine-rich domain Kratky plot 1.104 0 3 sRg
p(r)
Protein jagged-1 cysteine-rich domain pair distance distribution function Rg: 2.3 nm 0 Dmax: 8.2 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of Jagged1 cysteine-rich domain (CRD) 83 µM in 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH 7.4 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 4.0 m and (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.50 mg/ml was measured at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The expected molecular weight of the protein quoted above excludes the contribution of N-linked glycans. The total expected molecular weight of the protein in a monomeric state is 30 kDa (protein, 25 kDa plus glycans, 5 kDa). X-ray wavelength: UNKNOWN. Exposure time = UNKNOWN.

Protein jagged-1 cysteine-rich domain
Mol. type   Protein
Organism   Mus musculus
Olig. state   Unknown
Mon. MW   24.5 kDa
 
UniProt   Q9QXX0 (857-1067)
Sequence   FASTA