Comparison of angiopoietin-like protein 3 and 4 reveals structural and mechanistic similarities.

Gunn KH, Gutgsell AR, Xu Y, Johnson CV, Liu J, Neher SB, J Biol Chem :100312 (2021) Europe PMC

SASDJL8 – N-terminal Angiopoietin-like protein 3 Trimer

Angiopoietin-related protein 3
MWexperimental 56 kDa
MWexpected 81 kDa
VPorod 71 nm3
log I(s) 1.32×101 1.32×100 1.32×10-1 1.32×10-2
Angiopoietin-related protein 3 small angle scattering data  s, nm-1
ln I(s)
Angiopoietin-related protein 3 Guinier plot ln 1.33×101 Rg: 4.6 nm 0 (4.6 nm)-2 s2
Angiopoietin-related protein 3 Kratky plot 1.104 0 3 sRg
Angiopoietin-related protein 3 pair distance distribution function Rg: 4.9 nm 0 Dmax: 21.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Angiopoietin-related protein 3 DAMMIN model

Synchrotron SAXS data from solutions of angiopoietin-like protein 3 in 20 mM Tris-HCl pH 7.5, 400 mM NaCl, 2% glycerol, pH 7.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS, Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.07 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 80.00 μl sample at 6.2 mg/ml was injected at a 0.50 ml/min flow rate onto a Shodex KW-800 series column at 25°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Angiopoietin-related protein 3
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Trimer
Mon. MW   26.8 kDa
UniProt   Q9Y5C1 (16-224)
Sequence   FASTA