Structural basis of client specificity in mitochondrial membrane-protein chaperones.

Sučec I, Wang Y, Dakhlaoui O, Weinhäupl K, Jores T, Costa D, Hessel A, Brennich M, Rapaport D, Lindorff-Larsen K, Bersch B, Schanda P, Sci Adv 6(51) (2020) Europe PMC

SASDJQ4 – Mitochondrial import inner membrane translocase subunits TIM8-TIM13

Mitochondrial import inner membrane translocase subunit TIM8
Mitochondrial import inner membrane translocase subunit TIM13
MWexperimental 67 kDa
MWexpected 63 kDa
VPorod 136 nm3
log I(s) 7.15×101 7.15×100 7.15×10-1 7.15×10-2
Mitochondrial import inner membrane translocase subunit TIM8 Mitochondrial import inner membrane translocase subunit TIM13 small angle scattering data  s, nm-1
ln I(s)
Mitochondrial import inner membrane translocase subunit TIM8 Mitochondrial import inner membrane translocase subunit TIM13 Guinier plot ln 7.15×101 Rg: 3.1 nm 0 (3.1 nm)-2 s2
(sRg)2I(s)/I(0)
Mitochondrial import inner membrane translocase subunit TIM8 Mitochondrial import inner membrane translocase subunit TIM13 Kratky plot 1.104 0 √3 sRg
Dmax: 10.9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Mitochondrial import inner membrane translocase subunit TIM8 Mitochondrial import inner membrane translocase subunit TIM13 CUSTOM IN-HOUSE model
Mitochondrial import inner membrane translocase subunit TIM8 Mitochondrial import inner membrane translocase subunit TIM13 CUSTOM IN-HOUSE model
Mitochondrial import inner membrane translocase subunit TIM8 Mitochondrial import inner membrane translocase subunit TIM13 CUSTOM IN-HOUSE model
Mitochondrial import inner membrane translocase subunit TIM8 Mitochondrial import inner membrane translocase subunit TIM13 CUSTOM IN-HOUSE model
Mitochondrial import inner membrane translocase subunit TIM8 Mitochondrial import inner membrane translocase subunit TIM13 CUSTOM IN-HOUSE model
Mitochondrial import inner membrane translocase subunit TIM8 Mitochondrial import inner membrane translocase subunit TIM13 CUSTOM IN-HOUSE model
Mitochondrial import inner membrane translocase subunit TIM8 Mitochondrial import inner membrane translocase subunit TIM13 CUSTOM IN-HOUSE model
Mitochondrial import inner membrane translocase subunit TIM8 Mitochondrial import inner membrane translocase subunit TIM13 CUSTOM IN-HOUSE model
Mitochondrial import inner membrane translocase subunit TIM8 Mitochondrial import inner membrane translocase subunit TIM13 CUSTOM IN-HOUSE model
Mitochondrial import inner membrane translocase subunit TIM8 Mitochondrial import inner membrane translocase subunit TIM13 CUSTOM IN-HOUSE model
Synchrotron SAXS data from solutions of mitochondrial import inner membrane translocase subunits TIM8-TIM13 in 50 mM Tris, 150 mM NaCl, pH 7.4 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.50 mg/ml was measured at 25°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The experimental molecular weight is the one from the Porod volume.

Mitochondrial import inner membrane translocase subunit TIM8
Mol. type   Protein
Organism   Saccharomyces cerevisiae
Olig. state   Trimer
Mon. MW   9.8 kDa
 
UniProt   P57744 (1-87)
Sequence   FASTA
 
Mitochondrial import inner membrane translocase subunit TIM13
Mol. type   Protein
Organism   Saccharomyces cerevisiae
Olig. state   Trimer
Mon. MW   11.2 kDa
 
UniProt   P53299 (2-105)
Sequence   FASTA