SAXS data for Chitin-binding protein (CbpD) from Pseudomonas aeruginosa

Henrik Vinther Sørensen.

SASDJQ5 – Chitin-binding protein CbpD (Pseudomonas aeruginosa) at 24 °C

Chitin-binding protein CbpD
MWI(0) 38 kDa
MWexpected 39 kDa
VPorod 50 nm3
log I(s) 2.57×10-2 2.57×10-3 2.57×10-4 2.57×10-5
Chitin-binding protein CbpD small angle scattering data  s, nm-1
ln I(s)
Chitin-binding protein CbpD Guinier plot ln 2.57×10-2 Rg: 3.3 nm 0 (3.3 nm)-2 s2
Chitin-binding protein CbpD Kratky plot 1.104 0 3 sRg
Chitin-binding protein CbpD pair distance distribution function Rg: 3.5 nm 0 Dmax: 12.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Chitin-binding protein CbpD OTHER model

log I(s)
 s, nm-1
Chitin-binding protein CbpD DAMMIF model
Chitin-binding protein CbpD DAMFILT model

SAXS data from solutions of CbpD in 15 mM Tris-HCl 150 mM NaCl, pH 7.5 were collected using a Bruker Nanostar instrument with an InCoatec Cu microsource (RECX, University of Oslo, Norway) equipped with a Våntec-2000 detector at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.18 mg/ml was measured at 24°C. Two successive 3600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

For the dummy-atom model representations: An individual DAMMIF model with the corresponding fit to the data is displayed along with a damfilt representation generated from the spatial alignment of nine individual models.

Chitin-binding protein CbpD (CbpD)
Mol. type   Protein
Organism   Pseudomonas aeruginosa
Olig. state   Monomer
Mon. MW   39.2 kDa
UniProt   Q9I589 (26-389)
Sequence   FASTA