SAXS data for Chitin-binding protein (CbpD) from Pseudomonas aeruginosa

Henrik Vinther Sørensen.

SASDJR5 – Chitin-binding protein CbpD (Pseudomonas aeruginosa) at 37°C

Chitin-binding protein CbpD
MWexperimental 39 kDa
MWexpected 39 kDa
VPorod 63 nm3
log I(s) 6.44×10-2 6.44×10-3 6.44×10-4 6.44×10-5
Chitin-binding protein CbpD small angle scattering data  s, nm-1
ln I(s)
Chitin-binding protein CbpD Guinier plot ln 6.44×10-2 Rg: 3.5 nm 0 (3.5 nm)-2 s2
(sRg)2I(s)/I(0)
Chitin-binding protein CbpD Kratky plot 1.104 0 3 sRg
Dmax: 15 nm

Data validation


There are no models related to this curve.

SAXS data from solutions of CbpD in 15 mM Tris-HCl 150 mM NaCl, pH 7.5 were collected using a Bruker Nanostar instrument with an InCoatec Cu microsource (RECX, University of Oslo, Norway) equipped with a Våntec-2000 detector at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.18 mg/ml was measured at 37°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

X-ray exposure time: UNKNOWN

Chitin-binding protein CbpD (CbpD)
Mol. type   Protein
Organism   Pseudomonas aeruginosa
Olig. state   Monomer
Mon. MW   39.2 kDa
 
UniProt   Q9I589 (26-389)
Sequence   FASTA