| MWexperimental | 36 | kDa |
| MWexpected | 33 | kDa |
| VPorod | 34 | nm3 |
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log I(s)
6.36×100
6.36×10-1
6.36×10-2
6.36×10-3
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s, nm-1
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Aromatic residues, Tyr780, His782, Tyr796, Tyr801, located on PKD2 domain of Hathewaya histolytica collagenase, ColH, are involved in the 10-fold enhancement of binding affinity for the enzyme towards collagen fibril but not to mini-collagen
Perry Caviness.SASDJU6 – Collagenase H C-terminal non-catalytic segments Polycystic Kidney disease domain 1 (PKD1), Polycystic Kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) with Tyr689Ser, Phe712Ser, Tyr780Ser, His782Ser, Tyr796Ser and Tyr801Ser
Collagenase ColH (Polycystic kidney disease 1 (PKD1), Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) with Tyr689Ser, Phe712Ser, Tyr780Ser, His782Ser, Tyr796Ser and Tyr801)|
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Fits and models
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Synchrotron SAXS
data from solutions of
Collagenase H C-terminal non-catalytic segments Polycystic Kidney disease domain 1 (PKD1), Polycystic Kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) with Tyr689Ser, Phe712Ser, Tyr780Ser, His782Ser, Tyr796Ser and Tyr801Ser
in
50 mM Tris, 1 mM CaCl2, pH 7.5
were collected
on the
12.3.1 (SIBYLS) beam line
at the Advanced Light Source (ALS) storage ring
(Berkeley, CA, USA)
using a Pilatus3 X 2M detector
at a sample-detector distance of 1.5 m and
at a wavelength of λ = 0.1127 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 2 and 4 mg/ml were measured
at 10°C.
33 successive
0.300 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
Storage temperature = UNKNOWN |
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