Synchrotron SAXS
data from solutions of
X-ray repair cross-complementing proteins 5ΔCTR and 6
in
50 mM Hepes, 50 mM KCl, 5 mM MgCl2, 5% glycerol and 0.2 mM DTT, pH 7.5
were collected
on the
12.3.1 (SIBYLS) beam line
at the Advanced Light Source (ALS) storage ring
(Berkeley, CA, USA)
using a Pilatus3 X 2M detector
at a sample-detector distance of 2.1 m and
at a wavelength of λ = 0.1127 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 55.00 μl sample
at 5 mg/ml was injected at a 0.50 ml/min flow rate
onto a Shodex KW-800 series column
at 20°C.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
|
X-ray repair cross-complementing protein 6
(KU70)
|
Mol. type |
|
Protein |
Organism |
|
Homo sapiens |
Olig. state |
|
Monomer |
Mon. MW |
|
69.8 kDa |
|
UniProt |
|
P12956
(1-609)
|
Sequence |
|
FASTA |
|
X-ray repair cross-complementing protein 5 ΔCTR
(KU80ΔCTR)
|
Mol. type |
|
Protein |
Organism |
|
Homo sapiens |
Olig. state |
|
Monomer |
Mon. MW |
|
64.1 kDa |
|
UniProt |
|
P13010
(1-565)
|
Sequence |
|
FASTA |
|
|