Synchrotron SAXS data from solutions of EcoKMcrA restriction endonuclease bound to 12bp oligoduplex DNA in 20 mM Tris–HCl pH 7.5, 200 mM KCl, 0.1 mM EDTA, 0.01% (w/v) sodium azide and 1 mM DTT, were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.70 mg/ml was measured at 20°C. 18 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
EcoKMcrA (as monomeric protein chain) ratio to DNA oligoduplex in the sample was 1:1. NOTE! The DNA used in the models are homologous, and do not necessarily correspond in sequence, to the deposited DNA sequence of the complex analysed using SAXS.
|
cognate hemimethylated 12-bp oligoduplex
(12_5mC/12_C)
|
Mol. type |
|
DNA |
Olig. state |
|
Dimer |
Mon. MW |
|
7.4 kDa |
Sequence |
|
FASTA |
|
5-methylcytosine-specific restriction enzyme A
(EcoKMcrA)
|
Mol. type |
|
Protein |
Organism |
|
Escherichia coli |
Olig. state |
|
Dimer |
Mon. MW |
|
32.3 kDa |
|
UniProt |
|
P24200
(1-277)
|
Sequence |
|
FASTA |
|
|