Synchrotron SAXS
data from solutions of
DNA-dependent protein kinase catalytic subunit (DNA-PKcs)
in
50 mM Tris-HCl, 100 mM NaCl, 5% glycerol, 0.01% sodium azide, pH 7.5
were collected
on the
12.3.1 (SIBYLS) beam line
at the Advanced Light Source (ALS) storage ring
(Berkeley, CA, USA)
using a MAR 165 CCD detector
at a sample-detector distance of 1.5 m and
at a wavelength of λ = 0.103 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample
at 10 mg/ml was injected at a 0.25 ml/min flow rate
onto a Shodex KW403 column
at 20°C.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
|
DNA-dependent protein kinase catalytic subunit
(DNA-PKcs)
|
Mol. type |
|
Protein |
Organism |
|
Homo sapiens |
Olig. state |
|
Monomer |
Mon. MW |
|
468.3 kDa |
|
UniProt |
|
P78527
(10-4128)
|
Sequence |
|
FASTA |
|
|