Synchrotron SAXS data from solutions of EcoKMcrA restriction endonuclease (N-terminal domain) in 20 mM Tris–HCl pH 7.5, 200 mM KCl, 0.1 mM EDTA, 0.01% (w/v) sodium azide, 1 mM DTT, were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.3 and 3.5 mg/ml were measured at 20°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Prone to aggregation
|
5-methylcytosine-specific restriction enzyme A (N-terminal domain)
(EcoKMcrA-N)
|
Mol. type |
|
Protein |
Organism |
|
Escherichia coli |
Olig. state |
|
Monomer |
Mon. MW |
|
20.6 kDa |
|
UniProt |
|
P24200
(1-175)
|
Sequence |
|
FASTA |
|
|