Structural analysis of the SRP Alu domain from Plasmodium falciparum reveals a non-canonical open conformation.

Soni K, Kempf G, Manalastas-Cantos K, Hendricks A, Flemming D, Guizetti J, Simon B, Frischknecht F, Svergun DI, Wild K, Sinning I, Commun Biol 4(1):600 (2021) Europe PMC

SASDK34 – Full length SRP Alu RNA from Plasmodium falciparum

Full-length SRP Alu RNA
MWexperimental 38 kDa
MWexpected 38 kDa
VPorod 63 nm3
log I(s) 6.56×101 6.56×100 6.56×10-1 6.56×10-2
Full-length SRP Alu RNA small angle scattering data  s, nm-1
ln I(s)
Full-length SRP Alu RNA Guinier plot ln 6.57×101 Rg: 3.3 nm 0 (3.3 nm)-2 s2
(sRg)2I(s)/I(0)
Full-length SRP Alu RNA Kratky plot 1.104 0 3 sRg
p(r)
Full-length SRP Alu RNA pair distance distribution function Rg: 3.3 nm 0 Dmax: 11.8 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Full-length SRP Alu RNA DAMMIN model

Synchrotron SAXS data from solutions of full length SRP Alu RNA in 20 mM HEPES pH 7.5, 150 mM NaCl, 10 mM MgCl2, 10 mM KCl, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.80 mg/ml was measured at 20°C. 20 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Full-length SRP Alu RNA
Mol. type   RNA
Organism   Plasmodium falciparum
Olig. state   Monomer
Mon. MW   37.9 kDa
Sequence   FASTA