Structural insights into kinetoplastid coronin oligomerization domain and F-actin interaction

Parihar P, Singh A, Karade S, Sahasrabuddhe A, Pratap J, Current Research in Structural Biology (2021) DOI

SASDK56 – Cell cycle associated protein MOB1, putative, from Leishmania donovani

Cell cycle associated protein MOB1, putative
MWI(0) 23 kDa
MWexpected 27 kDa
VPorod 59 nm3
log I(s) 2.43×104 2.43×103 2.43×102 2.43×101
Cell cycle associated protein MOB1, putative small angle scattering data  s, nm-1
ln I(s)
Cell cycle associated protein MOB1, putative Guinier plot ln 2.44×104 Rg: 2.1 nm 0 (2.1 nm)-2 s2
(sRg)2I(s)/I(0)
Cell cycle associated protein MOB1, putative Kratky plot 1.104 0 √3 sRg
p(r)
Cell cycle associated protein MOB1, putative pair distance distribution function Rg: 2.1 nm 0 Dmax: 5.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Cell cycle associated protein MOB1, putative GASBOR model
SAXS data from solutions of MOB1 from Leishmania donovani in 100 mM Tris-HCl, 100 mM NaCl, pH 7.4 were collected using an Anton Paar SAXSpace instrument (CSIR-Central Drug Research Institute, India) equipped with a Mythen2 R 1K detector at a sample-detector distance of 0.3 m and at a wavelength of λ = 0.148 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 7.00 mg/ml was measured at 10°C. Two successive 120 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Cell cycle associated protein MOB1, putative (MOB1)
Mol. type   Protein
Organism   Leishmania donovani (strain BPK282A1)
Olig. state   Monomer
Mon. MW   26.7 kDa
 
UniProt   E9B8V9 (1-223)
Sequence   FASTA