BusR senses bipartite DNA binding motifs by a unique molecular ruler architecture.

Bandera AM, Bartho J, Lammens K, Drexler DJ, Kleinschwärzer J, Hopfner KP, Witte G, Nucleic Acids Res (2021) Europe PMC

SASDK84 – Streptococcus agalactiae transcription factor BusR

Transcriptional repressor BusR
MWexperimental 114 kDa
MWexpected 95 kDa
VPorod 168 nm3
log I(s) 1.71×104 1.71×103 1.71×102 1.71×101
Transcriptional repressor BusR small angle scattering data  s, nm-1
ln I(s)
Transcriptional repressor BusR Guinier plot ln 1.72×104 Rg: 4.4 nm 0 (4.4 nm)-2 s2
(sRg)2I(s)/I(0)
Transcriptional repressor BusR Kratky plot 1.104 0 3 sRg
p(r)
Transcriptional repressor BusR pair distance distribution function Rg: 4.5 nm 0 Dmax: 13.9 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of BusR in 200mM NaCl, 30 mM HEPES , 3% (v/v) glycerol, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123981 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 6 mg/ml was injected at a 0.25 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column at 20°C. 1800 successive 0.495 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Transcriptional repressor BusR (SgaBusR)
Mol. type   Protein
Organism   Streptococcus agalactiae
Olig. state   Tetramer
Mon. MW   23.9 kDa
 
UniProt   Q8E533 (1-213)
Sequence   FASTA