BusR senses bipartite DNA binding motifs by a unique molecular ruler architecture.

Bandera AM, Bartho J, Lammens K, Drexler DJ, Kleinschwärzer J, Hopfner KP, Witte G, Nucleic Acids Res (2021) Europe PMC

SASDK94 – Streptococcus agalactiae transcription factor BusR dsDNA complex

Transcriptional repressor BusR
BusR Recognition sequence
MWexperimental 125 kDa
MWexpected 124 kDa
VPorod 210 nm3
log I(s) 1.27×104 1.27×103 1.27×102 1.27×101
Transcriptional repressor BusR BusR Recognition sequence small angle scattering data  s, nm-1
ln I(s)
Transcriptional repressor BusR BusR Recognition sequence Guinier plot ln 1.28×104 Rg: 4.3 nm 0 (4.3 nm)-2 s2
(sRg)2I(s)/I(0)
Transcriptional repressor BusR BusR Recognition sequence Kratky plot 1.104 0 3 sRg
p(r)
Transcriptional repressor BusR BusR Recognition sequence pair distance distribution function Rg: 4.4 nm 0 Dmax: 14.2 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Transcriptional repressor BusR BusR Recognition sequence OTHER model

Synchrotron SAXS data from a BusR-dsDNA complex in 20mM HEPES, pH6.5, 100mM NaCl, 3% glycerol (v/v) were collected on the EMBL P12 beam line at PETRA III storage ring (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 10 mg/ml was injected at a 0.6 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 2400 successive 1 second frames were collected through the entire SEC run; 66 data frames measured through the sample elution peak were used to generate the displayed profile. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Transcriptional repressor BusR (SgaBusR)
Mol. type   Protein
Organism   Streptococcus agalactiae
Olig. state   Tetramer
Mon. MW   23.9 kDa
 
UniProt   Q8E533 (1-213)
Sequence   FASTA
 
BusR Recognition sequence (pAB1SAXS)
Mol. type   DNA
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   28.4 kDa
Sequence   FASTA
 
PDB code   7B5Y