Nucleosome scaffolding by Brd4 tandem bromodomains in acetylation-dependent chromatin compartmentalization

Olp M, Jackson V, Smith B, (2019) DOI

SASDK97 – Bromodomain-containing protein 2, BRD2, tandem bromodomains (2 mg/ml)

Bromodomain-containing protein 2
MWexperimental 32 kDa
MWexpected 43 kDa
VPorod 100 nm3
log I(s) 8.91×100 8.91×10-1 8.91×10-2 8.91×10-3
Bromodomain-containing protein 2 small angle scattering data  s, nm-1
ln I(s)
Bromodomain-containing protein 2 Guinier plot ln 8.92×100 Rg: 4.6 nm 0 (4.6 nm)-2 s2
(sRg)2I(s)/I(0)
Bromodomain-containing protein 2 Kratky plot 1.104 0 √3 sRg
p(r)
Bromodomain-containing protein 2 pair distance distribution function Rg: 4.7 nm 0 Dmax: 17.0 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Bromodomain-containing protein 2, BRD2, tandem bromodomains (2 mg/ml) in 25 mM HEPES, 150 mM NaCl, and 2% glycerol, pH 7.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS) storage ring (Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.128 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 10°C. 32 successive 0.300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Bromodomain-containing protein 2 (BRD2)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   43.4 kDa
 
UniProt   P25440 (71-455)
Sequence   FASTA