Spatial Organization of Dps and DNA-Dps Complexes.

Dubrovin EV, Dadinova LA, Petoukhov MV, Yu Soshinskaya E, Mozhaev AA, Klinov DV, Schäffer TE, Shtykova EV, Batishchev OV, J Mol Biol :166930 (2021) Europe PMC

SASDKF4 – DNA binding protein of starvation (DPS)

DNA protection during starvation protein
MWexperimental 224 kDa
MWexpected 225 kDa
VPorod 314 nm3
log I(s) 2.36×10-1 2.36×10-2 2.36×10-3 2.36×10-4
DNA protection during starvation protein small angle scattering data  s, nm-1
ln I(s)
DNA protection during starvation protein Guinier plot ln 2.37×10-1 Rg: 3.9 nm 0 (3.9 nm)-2 s2
(sRg)2I(s)/I(0)
DNA protection during starvation protein Kratky plot 1.104 0 3 sRg
p(r)
DNA protection during starvation protein pair distance distribution function Rg: 3.8 nm 0 Dmax: 14 nm

Data validation


Fits and models


log I(s)
 s, nm-1
DNA protection during starvation protein DAMMIN model

log I(s)
 s, nm-1
DNA protection during starvation protein CORAL model

Synchrotron SAXS data from solutions of DNA binding protein of starvation (DPS) in 50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, pH 8 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 6M detector at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.40 mg/ml was measured at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

DNA protection during starvation protein (DPS)
Mol. type   Protein
Organism   Escherichia coli
Olig. state   Dodecamer
Mon. MW   18.7 kDa
 
UniProt   A7ZJM7 (1-167)
Sequence   FASTA