Solution Structure, Self-Assembly, and Membrane Interactions of the Matrix Protein from Newcastle Disease Virus at Neutral and Acidic pH

Shtykova E, Petoukhov M Dadinova L, Fedorova N, Tashkin V, Timofeeva T, Ksenofontov A, Loshkarev N, Baratova L, Jeffries C, Svergun D, Batishchev O, García-Sastre A, Journal of Virology 93(6) (2019) DOI

SASDKP2 – Matrix protein from Newcastle disease virus at acidic pH

Matrix protein
MWexperimental 81 kDa
MWexpected 79 kDa
log I(s) 1.49×103 1.49×102 1.49×101 1.49×100
Matrix protein small angle scattering data  s, nm-1
ln I(s)
Matrix protein Guinier plot ln 1.49×103 Rg: 3.3 nm 0 (3.3 nm)-2 s2
(sRg)2I(s)/I(0)
Matrix protein Kratky plot 1.104 0 √3 sRg

Data validation

Fits and models

log I(s)
 s, nm-1
Matrix protein PDB (PROTEIN DATA BANK) model
Matrix protein PISA model
Matrix protein CUSTOM IN-HOUSE model
Synchrotron SAXS data from solutions of Matrix protein from Newcastle disease virus at acidic pH in STE buffer 100 mM NaCl, 10 mM Tris-HCl, and 1 mM EDTA, pH 4 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.20 mg/ml was measured at 20°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Matrix protein
Mol. type   Protein
Organism   Newcastle disease virus (strain Chicken/Australia-Victoria/32)
Olig. state   Dimer
Mon. MW   39.7 kDa
 
UniProt   P11206
Sequence   FASTA
 
PDB ID   4G1L