Structure and dynamics of the quaternary hunchback mRNA translation repression complex.

Macošek J, Simon B, Linse JB, Jagtap PKA, Winter SL, Foot J, Lapouge K, Perez K, Rettel M, Ivanović MT, Masiewicz P, Murciano B, Savitski MM, Loedige I, Hub JS, Gabel F, Hennig J, Nucleic Acids Res 49(15):8866-8885 (2021) Europe PMC

SASDKR7 – Hunchback mRNA translation repression complex of Brat-NHL, Pum-HD, Nanos-ZnF and hb NRE2 RNA

Brain tumor protein
Maternal protein pumilio
Protein nanos
hunchback mRNA Nanos Response Element 2
MWexperimental 85 kDa
MWexpected 88 kDa
VPorod 114 nm3
log I(s) 1.54×104 1.54×103 1.54×102 1.54×101
Brain tumor protein Maternal protein pumilio Protein nanos hunchback mRNA Nanos Response Element 2 small angle scattering data  s, nm-1
ln I(s)
Brain tumor protein Maternal protein pumilio Protein nanos hunchback mRNA Nanos Response Element 2 Guinier plot ln 1.55×104 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
Brain tumor protein Maternal protein pumilio Protein nanos hunchback mRNA Nanos Response Element 2 Kratky plot 1.104 0 3 sRg
p(r)
Brain tumor protein Maternal protein pumilio Protein nanos hunchback mRNA Nanos Response Element 2 pair distance distribution function Rg: 3.8 nm 0 Dmax: 12.7 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of the hunchback mRNA translation repression complex (Brat-NHL, Pum-HD, Nanos-ZnF and hb NRE2 RNA) in 50 mM Tris, 150 mM NaCl, 1 mM DTT, 3% glycerol, pH 7.4 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 75.00 μl sample at 7.2 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 25°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted from the appropriate SEC peak data frames through the elution profile of the complex.

Brain tumor protein (Brat)
Mol. type   Protein
Organism   Drosophila melanogaster
Olig. state   Monomer
Mon. MW   31.9 kDa
 
UniProt   Q8MQJ9 (756-1037)
Sequence   FASTA
 
Maternal protein pumilio (Pum)
Mol. type   Protein
Organism   Drosophila melanogaster
Olig. state   Monomer
Mon. MW   38.4 kDa
 
UniProt   P25822 (1093-1426)
Sequence   FASTA
 
Protein nanos (Nos)
Mol. type   Protein
Organism   Drosophila melanogaster
Olig. state   Monomer
Mon. MW   10.6 kDa
 
UniProt   P25724 (301-392)
Sequence   FASTA
 
hunchback mRNA Nanos Response Element 2 (NRE2 RNA)
Mol. type   RNA
Organism   Drosophila melanogaster
Olig. state   Monomer
Mon. MW   7.4 kDa
Sequence   FASTA