The dimeric ectodomain of the alkali-sensing insulin receptor-related receptor (ectoIRR) has a droplike shape.

Shtykova EV, Petoukhov MV, Mozhaev AA, Deyev IE, Dadinova LA, Loshkarev NA, Goryashchenko AS, Bocharov EV, Jeffries CM, Svergun DI, Batishchev OV, Petrenko AG, J Biol Chem 294(47):17790-17798 (2019) Europe PMC

SASDKS3 – The dimeric ectodomain of the alkali-sensing insulin receptor–related receptor (ectoIRR) at pH7

Insulin receptor-related protein
MWexperimental 280 kDa
MWexpected 201 kDa
VPorod 444 nm3
log I(s) 1.63×104 1.63×103 1.63×102 1.63×101
Insulin receptor-related protein small angle scattering data  s, nm-1
ln I(s)
Insulin receptor-related protein Guinier plot ln 1.64×104 Rg: 5.4 nm 0 (5.4 nm)-2 s2
Insulin receptor-related protein Kratky plot 1.104 0 3 sRg
Insulin receptor-related protein pair distance distribution function Rg: 5.4 nm 0 Dmax: 19.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Insulin receptor-related protein CORAL model

Synchrotron SAXS data from solutions of the dimeric ectodomain of the alkali-sensing insulin receptor–related receptor (ectoIRR) in 150 mM NaCl, 20 mM Tris, pH 7 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 2M detector at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample detector distance = UNKNOWN

Insulin receptor-related protein (ectoIRR)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   100.7 kDa
UniProt   P14616 (28-918)
Sequence   FASTA