pUL21 is a viral phosphatase adaptor that promotes herpes simplex virus replication and spread.

Benedyk TH, Muenzner J, Connor V, Han Y, Brown K, Wijesinghe KJ, Zhuang Y, Colaco S, Stoll GA, Tutt OS, Svobodova S, Svergun DI, Bryant NA, Deane JE, Firth AE, Jeffries CM, Crump CM, Graham SC, PLoS Pathog 17(8):e1009824 (2021) Europe PMC

SASDKW8 – Full-length pUL21 with C-terminal His-tag

Tegument protein UL21
MWexperimental 60 kDa
MWexpected 59 kDa
VPorod 90 nm3
log I(s) 2.79×10-2 2.79×10-3 2.79×10-4 2.79×10-5
Tegument protein UL21 small angle scattering data  s, nm-1
ln I(s)
Tegument protein UL21 Guinier plot ln 2.79×10-2 Rg: 4.2 nm 0 (4.2 nm)-2 s2
Tegument protein UL21 Kratky plot 1.104 0 3 sRg
Tegument protein UL21 pair distance distribution function Rg: 4.5 nm 0 Dmax: 18.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Full-length pUL21 with C-terminal His-tag Rg histogram Rg, nm
Tegument protein UL21 EOM/RANCH model
Tegument protein UL21 EOM/RANCH model
Tegument protein UL21 EOM/RANCH model
Tegument protein UL21 EOM/RANCH model
Tegument protein UL21 EOM/RANCH model

Synchrotron SAXS data from solutions of full-length pUL21 in 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, were collected on the EMBL-P12 bioSAXS beam line at Petra III (DESY Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.1 and 1.7 mg/ml were measured at 20°C. 47 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The reported experimental SAS data were obtained by averaging data sets measured from two dilutions: 1.11 mg/ml and 1.66 mg/ml.

Tegument protein UL21
Mol. type   Protein
Organism   Human alphaherpesvirus 1 strain KOS
Olig. state   Monomer
Mon. MW   58.7 kDa
UniProt   P10205 (1-535)
Sequence   FASTA