Structure and dynamics of Toll immunoreceptor activation in the mosquito Aedes aegypti.

Saucereau Y, Wilson TH, Tang MCK, Moncrieffe MC, Hardwick SW, Chirgadze DY, Soares SG, Marcaida MJ, Gay NJ, Gangloff M, Nat Commun 13(1):5110 (2022) Europe PMC

SASDKX8 – Aedes aegypti Toll5A receptor ectodomain (T5A)

Toll-like receptor
MWexperimental 200 kDa
MWexpected 175 kDa
VPorod 370 nm3
log I(s) 2.89×10-1 2.89×10-2 2.89×10-3 2.89×10-4
Toll-like receptor small angle scattering data  s, nm-1
ln I(s)
Toll-like receptor Guinier plot ln 2.90×10-1 Rg: 5.4 nm 0 (5.4 nm)-2 s2
(sRg)2I(s)/I(0)
Toll-like receptor Kratky plot 1.104 0 √3 sRg
p(r)
Toll-like receptor pair distance distribution function Rg: 5.5 nm 0 Dmax: 20.5 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Aedes aegypti Toll5A receptor ectodomain (T5A) in 50 mM NaCl, 50 mM Tris-HCl, pH 7.5 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 55.00 μl sample at 5 mg/ml was injected at a 0.075 ml/min flow rate onto a GE Superdex 6 Increase 3.2/300 column at 20°C. 29 successive 3 second frames were collected through the SEC elution peak of the sample. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN

Toll-like receptor (T5A)
Mol. type   Protein
Organism   Aedes aegypti
Olig. state   Dimer
Mon. MW   87.6 kDa
 
UniProt   A0A6I8TEX2 (28-789)
Sequence   FASTA