Toll5A and Spz1C

Monique Gangloff.

SASDKX8 – Aedes aegypti Toll5A receptor ectodomain (T5A)

Toll-like receptor
MWexperimental 200 kDa
MWexpected 175 kDa
VPorod 370 nm3
log I(s) 2.89×10-1 2.89×10-2 2.89×10-3 2.89×10-4
Toll-like receptor small angle scattering data  s, nm-1
ln I(s)
Toll-like receptor Guinier plot ln 2.90×10-1 Rg: 5.4 nm 0 (5.4 nm)-2 s2
Toll-like receptor Kratky plot 1.104 0 3 sRg
Toll-like receptor pair distance distribution function Rg: 5.5 nm 0 Dmax: 20.5 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Aedes aegypti Toll5A receptor ectodomain (T5A) in 50 mM NaCl, 50 mM Tris-HCl, pH 7.5 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 55.00 μl sample at 5 mg/ml was injected at a 0.075 ml/min flow rate onto a GE Superdex 6 Increase 3.2/300 column at 20°C. 29 successive 3 second frames were collected through the SEC elution peak of the sample. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN

Toll-like receptor (T5A)
Mol. type   Protein
Organism   Aedes aegypti
Olig. state   Dimer
Mon. MW   87.6 kDa
UniProt   A0A6I8TEX2 (28-789)
Sequence   FASTA