Toll5A and Spz1C

Monique Gangloff.

SASDKY8 – Aedes aegypti Toll5A ectodomain in complex with Spz1C Cys-knot domain

Toll-like receptor
AAEL013433-PA (Cystine-knot_cytokine)
MWexperimental 270 kDa
MWexpected 201 kDa
VPorod 567 nm3
log I(s) 2.94×10-1 2.94×10-2 2.94×10-3 2.94×10-4
Toll-like receptor AAEL013433-PA (Cystine-knot_cytokine) small angle scattering data  s, nm-1
ln I(s)
Toll-like receptor AAEL013433-PA (Cystine-knot_cytokine) Guinier plot ln 2.94×10-1 Rg: 5.5 nm 0 (5.5 nm)-2 s2
(sRg)2I(s)/I(0)
Toll-like receptor AAEL013433-PA (Cystine-knot_cytokine) Kratky plot 1.104 0 3 sRg
p(r)
Toll-like receptor AAEL013433-PA (Cystine-knot_cytokine) pair distance distribution function Rg: 5.7 nm 0 Dmax: 21.5 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of the Aedes aegypti Toll5A ectodomain in complex with Spz1C Cys-knot domain in 50 mM NaCl, 50 mM Tris-HCl, pH 7.5 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 55.00 μl sample at 5 mg/ml was injected at a 0.075 ml/min flow rate onto a GE Superdex 6 Increase 3.2/300 column at 20°C. 31 successive 3 second frames were collected through the SEC elution peak of the sample. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The Toll5A-Spz1C complex was concentrated to 5 mg/mL in 50mM NaCl, 50 mM Tris-HCl pH7.5 and injected into a Superose 6 Increase 3.2 equilibrated in the same buffer at a flow rate of 0.075 ml/min. Data points were collected every 3 sec.

Toll-like receptor (T5A)
Mol. type   Protein
Organism   Aedes aegypti
Olig. state   Dimer
Mon. MW   87.6 kDa
 
UniProt   A0A6I8TEX2 (28-789)
Sequence   FASTA
 
AAEL013433-PA (Cystine-knot_cytokine)
Mol. type   Protein
Organism   Aedes aegypti
Olig. state   Dimer
Mon. MW   13.0 kDa
 
UniProt   Q16J57 (142-243)
Sequence   FASTA