SAXS data from solutions of the minimal trans VS ribozyme in 50 mM MES, 50 mM KCl, 20 mM MgCl2, pH 6.5 were collected using a Xenocs BioXolver L with MetalJet equipped with a Pilatus3 R 300K detector (Université de Montréal, Montréal, Canada) at a sample-detector distance of 0.6 m and at a wavelength of λ = 0.134 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 0.5 mg/ml were measured at 20°C. 10 successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Neurospora Varkud Satellite minimal trans ribozyme
(VS ribozyme TR4P)