| MWexperimental | 176 | kDa |
| MWexpected | 108 | kDa |
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log I(s)
8.49×10-2
8.49×10-3
8.49×10-4
8.49×10-5
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s, nm-1
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A structural study of the cytoplasmic chaperone effect of 14-3-3 proteins on Ataxin-1.
Leysen S Jane Burnley R, Rodriguez E, Milroy LG, Soini L, Adamski CJ, Nitschke L, Davis R, Obsil T, Brunsveld L, Crabbe T, Yahya Zoghbi H, Ottmann C, Martin Davis J, J Mol Biol :167174 (2021) Europe PMCSASDL46 – 14-3-3zeta AXH-C complex
14-3-3 protein zeta/deltaAtaxin-1 AXH-C
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Fits and models
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Synchrotron SAXS
data from solutions of
14-3-3zeta AXH-C complex
in
20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH 7.5
were collected
on the
B21 beam line
at the Diamond Light Source storage ring
(Didcot, UK)
using a Pilatus 2M detector
at a sample-detector distance of 4.0 m and
at a wavelength of λ = 0.099987 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample
at 11 mg/ml was injected
onto a Shodex KW400 series column
at 25°C.
620 successive
3 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Storage temperature = UNKNOWN. Flow rate = UNKNOWN |
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