Structural, thermodynamic and functional studies of human 71 kDa heat shock cognate protein (HSPA8/hHsc70)

Silva N, de Camargo Rodrigues L, Dores-Silva P, Montanari C, Ramos C, Barbosa L, Borges J, Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics :140719 (2021) DOI

SASDL52 – Human Heat Shock 70 kDa Protein 1A (Hsp70-1A/HSPA1A)

Heat shock 70 kDa protein 1A
MWexperimental 67 kDa
MWexpected 70 kDa
VPorod 138 nm3
log I(s) 5.97×105 5.97×104 5.97×103 5.97×102
Heat shock 70 kDa protein 1A small angle scattering data  s, nm-1
ln I(s)
Heat shock 70 kDa protein 1A Guinier plot ln 5.97×105 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
Heat shock 70 kDa protein 1A Kratky plot 1.104 0 3 sRg
p(r)
Heat shock 70 kDa protein 1A pair distance distribution function Rg: 3.6 nm 0 Dmax: 11 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Heat shock 70 kDa protein 1A DAMMIN model

Synchrotron SAXS data from solutions of Human Heat Shock 70 kDa Protein 1A (Hsp70-1A/HSPA1A) in 25 mM Tris HCl, 50 mM NaCl, 5 mM Sodium Phosphate, 5 mM KCl, pH 7.5 were collected on the SAXS1 Beamline beam line at the Brazilian Synchrotron Light Laboratory storage ring (Campinas, Brazil) using a Pilatus 300K detector at a sample-detector distance of 1 m and at a wavelength of λ = 0.1488 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.50 mg/ml was measured at 27°C. 10 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Heat shock 70 kDa protein 1A (HSPA1A, Hsp70-1A)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   70.1 kDa
 
UniProt   P0DMV8 (1-586)
Sequence   FASTA