Cooperativity of UBA5-UFM1 complex formation

Sebastian Fuchs.

SASDL83 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 3 mg/mL + UFM1 0.75 mg/ml)

Ubiquitin-like modifier-activating enzyme 5
Ubiquitin fold modifer 1
MWexperimental 53 kDa
MWexpected 77 kDa
log I(s) 2.42×10-2 2.42×10-3 2.42×10-4 2.42×10-5
Ubiquitin-like modifier-activating enzyme 5 Ubiquitin fold modifer 1 small angle scattering data  s, nm-1
ln I(s)
Ubiquitin-like modifier-activating enzyme 5 Ubiquitin fold modifer 1 Guinier plot ln 2.42×10-2 Rg: 3.0 nm 0 (3.0 nm)-2 s2
(sRg)2I(s)/I(0)
Ubiquitin-like modifier-activating enzyme 5 Ubiquitin fold modifer 1 Kratky plot 1.104 0 3 sRg
p(r)
Ubiquitin-like modifier-activating enzyme 5 Ubiquitin fold modifer 1 pair distance distribution function Rg: 3.1 nm 0 Dmax: 12 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Ubiquitin-like modifier-activating enzyme 5 Ubiquitin fold modifer 1 SASREF model
Ubiquitin-like modifier-activating enzyme 5 Ubiquitin fold modifer 1 PDB model
Ubiquitin-like modifier-activating enzyme 5 Ubiquitin fold modifer 1 SASREF model

Synchrotron SAXS data from solutions of Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 3 mg/mL + UFM1 0.75 mg/ml) in 20 mM Tris, 50 mM NaCl, 2 mM DTT, pH 7.5 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123975 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 20°C. 28 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Ubiquitin-like modifier-activating enzyme 5 (UBA5)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   34.2 kDa
 
UniProt   Q9GZZ9 (57-346)
Sequence   FASTA
 
Ubiquitin fold modifer 1 (UFM1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   8.9 kDa
 
UniProt   P61960 (1-83)
Sequence   FASTA
 
PDB code   5IAA