| MWexperimental | 60 | kDa |
| MWexpected | 77 | kDa |
| VPorod | 100 | nm3 |
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log I(s)
2.67×10-2
2.67×10-3
2.67×10-4
2.67×10-5
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s, nm-1
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Structure and dynamics of UBA5-UFM1 complex formation showing new insights in the UBA5 activation mechanism
Fuchs S Kikhney A, Schubert R, Kaiser C, Liebau E, Svergun D, Betzel C, Perbandt M, Journal of Structural Biology :107796 (2021) DOISASDLA3 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 5 mg/mL + UFM1 1.25 mg/ml)
Ubiquitin-like modifier-activating enzyme 5Ubiquitin fold modifer 1
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Fits and models
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Synchrotron SAXS
data from solutions of
Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 5 mg/mL + UFM1 1.25 mg/ml)
in
20 mM Tris, 50 mM NaCl, 2 mM DTT, pH 7.5
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 6M detector
at a sample-detector distance of 3 m and
at a wavelength of λ = 0.123975 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 5.00 mg/ml was measured
at 20°C.
29 successive
0.100 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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